Journal: Brain Disorders

Article Title: Investigation for the involvement of microbial FliC and DING proteins in Alzheimer's Disease and Mild Cognitive Impairment and correlation with neurodegeneration and inflammation markers

doi: 10.1016/j.dscb.2023.100091

Figure Lengend Snippet: Fig. 4. Scatter plots of (a) calculated FliC levels against predicted FliC values, and (b) calculated DING proteins levels against predicted DING proteins levels, as analyzed by multivariable regression analysis. The graphs given represent the best-achieved models for the prediction of actual FliC and DING levels in CSF of all the donors enlisted in the current study, as affected by the levels of inflammatory markers COX-1 and COX-2, AD-related biomarkers Аβ42 and tau, and the microbial markers (a) DING or (b) FliC. Multilinear analysis was performed with the process of stepwise elimination, to evaluate the individual effect of the aforementioned markers. The calculated R squared values of the best-achieved models, their respective p values, and equations are provided in the respective plot. Statistical analysis was carried out by employing Graph Pad Prism 8.0 statistical software.

Article Snippet: Аnti-COX-1 (#160,108) and COX-2 (#160,107) rabbit polyclonal antibodies were from Cayman Chemical (Ann Arbor, MI, USA), while mouse monoclonal antibodies Anti-Аβ42 (#sc-28,365) and anti-tau (#AHB0042), were purchased from Santa Cruz (Dallas, TX, USA) and Invitrogen (Waltham, MA, USA), respectively.

Techniques: Software